The FlowMaster MITAS laser imaging system comprises a fully motorized 3-axis microscope stage with a high performance controller and a high precision microscope objective. For standard applications a 500mW DPSS laser for pulsed illumination, a high sensitive, dual frame-multiple exposure CCD camera is used. A system PC with built-in synchronisation unit controls the complete laser imaging system. LaVision’s modular DaVis software is used for advanced image acquisition and data analysis. The xyz(focus)-traverse system can be operated manually using a joy stick or the device control manager in DaVis. The light is delivered through an optical fiber to the microscope. A built-in pilot LED is used for target focusing. The filter cube exchange box allows a fast adaptation for different excitation and emission wavelengths. CCD camera, light source and synchronization are also under DaVis control.